Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.
Identifieur interne : 003E60 ( Main/Exploration ); précédent : 003E59; suivant : 003E61Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.
Auteurs : Bo Zhao [République populaire de Chine] ; Ning-Yi Jin ; Rui-Lin Wang ; Li-Shu Zhang ; Ying-Jiu ZhangSource :
- Viral immunology [ 0882-8245 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux, Cricetinae, Données de séquences moléculaires, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (administration et posologie), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Immunisation, Immunoglobuline G (sang), Lignée cellulaire, Lymphocytes T CD4+ (immunologie), Lymphocytes T CD8+ (immunologie), Lymphocytes T cytotoxiques (immunologie), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (administration et posologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Séquence d'acides aminés, Séquence nucléotidique, Vaccins à ADN (administration et posologie), Vaccins à ADN (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins à ADN.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Glycoprotéines membranaires, Lymphocytes T CD4+, Lymphocytes T CD8+, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Vaccins à ADN, Virus du SRAS.
- sang : Immunoglobuline G.
- Animaux, Anticorps antiviraux, Cricetinae, Données de séquences moléculaires, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Immunisation, Lignée cellulaire, Protéines de l'enveloppe virale, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral, Base Sequence, CD4-Positive T-Lymphocytes (immunology), CD8-Positive T-Lymphocytes (immunology), Cell Line, Cricetinae, Female, Immunization, Immunoglobulin G (blood), Membrane Glycoproteins (administration & dosage), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Molecular Sequence Data, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, T-Lymphocytes, Cytotoxic (immunology), Vaccines, DNA (administration & dosage), Vaccines, DNA (immunology), Viral Envelope Proteins (administration & dosage), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , administration & dosage : Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical , blood : Immunoglobulin G.
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical : Antibodies, Viral, Spike Glycoprotein, Coronavirus.
- immunology : CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic.
- prevention & control : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cricetinae, Female, Immunization, Mice, Mice, Inbred BALB C, Molecular Sequence Data.
Abstract
According to data in GenBank, a gene encoding SARS spike protein fragment 1 (S1) was synthesized. After recombination with an immunostimulatory sequence (ISS), the gene was cloned into the plasmid pIRES to produce pIRES-ISS-S1. On confirmation of the expression of S1 protein by indirect immunofluorescence assay (IFA), after the transfection of pIRES-ISS-S1 into BHK-21 cells, the DNA vaccine was repeatedly administrated to BALB/c mice. CD4+ and CD8+ spleen T lymphocytes were analyzed by flow cytometry (FCM) to evaluate T cell-mediated immune responses, the antigen-specific responses of T cells were evaluated by cytotoxic T lymphocyte (CTL) assay, and the level of IgG in antisera from immunized mice was determined by enzyme-linked immunosorbent assay. Results showed that the counts of spleen CD4+ and CD8+ T lymphocytes were increased, that the T cell-mediated immune responses showed antigen specificity, and that IgG was significantly induced with DNA vaccines pIRES-ISS-S1 and pIRES-S1 at titers of 1:320 and 1:160, respectively. These results are promising for the protective immunization of humans.
DOI: 10.1089/vim.2006.19.518
PubMed: 16987069
Affiliations:
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Le document en format XML
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<author><name sortKey="Wang, Rui Lin" sort="Wang, Rui Lin" uniqKey="Wang R" first="Rui-Lin" last="Wang">Rui-Lin Wang</name>
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<term>Animals</term>
<term>Antibodies, Viral</term>
<term>Base Sequence</term>
<term>CD4-Positive T-Lymphocytes (immunology)</term>
<term>CD8-Positive T-Lymphocytes (immunology)</term>
<term>Cell Line</term>
<term>Cricetinae</term>
<term>Female</term>
<term>Immunization</term>
<term>Immunoglobulin G (blood)</term>
<term>Membrane Glycoproteins (administration & dosage)</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Molecular Sequence Data</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>T-Lymphocytes, Cytotoxic (immunology)</term>
<term>Vaccines, DNA (administration & dosage)</term>
<term>Vaccines, DNA (immunology)</term>
<term>Viral Envelope Proteins (administration & dosage)</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux</term>
<term>Cricetinae</term>
<term>Données de séquences moléculaires</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (administration et posologie)</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Immunisation</term>
<term>Immunoglobuline G (sang)</term>
<term>Lignée cellulaire</term>
<term>Lymphocytes T CD4+ (immunologie)</term>
<term>Lymphocytes T CD8+ (immunologie)</term>
<term>Lymphocytes T cytotoxiques (immunologie)</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (administration et posologie)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Vaccins à ADN (administration et posologie)</term>
<term>Vaccins à ADN (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Vaccines, DNA</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Vaccines, DNA</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Viral</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Vaccins à ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Lymphocytes T CD4+</term>
<term>Lymphocytes T CD8+</term>
<term>Lymphocytes T cytotoxiques</term>
<term>Protéines de l'enveloppe virale</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Vaccins à ADN</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term>
<term>CD8-Positive T-Lymphocytes</term>
<term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
<term>T-Lymphocytes, Cytotoxic</term>
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<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
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<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Immunoglobuline G</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Cell Line</term>
<term>Cricetinae</term>
<term>Female</term>
<term>Immunization</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Molecular Sequence Data</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux</term>
<term>Cricetinae</term>
<term>Données de séquences moléculaires</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Immunisation</term>
<term>Lignée cellulaire</term>
<term>Protéines de l'enveloppe virale</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
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<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">According to data in GenBank, a gene encoding SARS spike protein fragment 1 (S1) was synthesized. After recombination with an immunostimulatory sequence (ISS), the gene was cloned into the plasmid pIRES to produce pIRES-ISS-S1. On confirmation of the expression of S1 protein by indirect immunofluorescence assay (IFA), after the transfection of pIRES-ISS-S1 into BHK-21 cells, the DNA vaccine was repeatedly administrated to BALB/c mice. CD4+ and CD8+ spleen T lymphocytes were analyzed by flow cytometry (FCM) to evaluate T cell-mediated immune responses, the antigen-specific responses of T cells were evaluated by cytotoxic T lymphocyte (CTL) assay, and the level of IgG in antisera from immunized mice was determined by enzyme-linked immunosorbent assay. Results showed that the counts of spleen CD4+ and CD8+ T lymphocytes were increased, that the T cell-mediated immune responses showed antigen specificity, and that IgG was significantly induced with DNA vaccines pIRES-ISS-S1 and pIRES-S1 at titers of 1:320 and 1:160, respectively. These results are promising for the protective immunization of humans.</div>
</front>
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<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><noCountry><name sortKey="Jin, Ning Yi" sort="Jin, Ning Yi" uniqKey="Jin N" first="Ning-Yi" last="Jin">Ning-Yi Jin</name>
<name sortKey="Wang, Rui Lin" sort="Wang, Rui Lin" uniqKey="Wang R" first="Rui-Lin" last="Wang">Rui-Lin Wang</name>
<name sortKey="Zhang, Li Shu" sort="Zhang, Li Shu" uniqKey="Zhang L" first="Li-Shu" last="Zhang">Li-Shu Zhang</name>
<name sortKey="Zhang, Ying Jiu" sort="Zhang, Ying Jiu" uniqKey="Zhang Y" first="Ying-Jiu" last="Zhang">Ying-Jiu Zhang</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Bo" sort="Zhao, Bo" uniqKey="Zhao B" first="Bo" last="Zhao">Bo Zhao</name>
</noRegion>
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